Fig. 4: ICAD reverses the inhibitory role of CAD on STING signaling.
From: CAD hijacks STING to impair antitumor immunity and radiotherapy efficacy of colorectal cancer
A Western blotting analyses of p-TBK1, TBK1, p-IRF3, and IRF3 levels in HCT116 and HT29 cells after ectopic overexpression of CAD and inhibitor of CAD (ICAD). B qPCR analyses of CCL5, CXCL10, and IFN-β mRNA in indicated cells at 24 h after irradiation. Error bars represent mean ± SD. C Immunoprecipitation and western blotting analyses of the interaction between STING and CAD upon overexpressing ICAD in HEK293T cells. D Immunoprecipitation and western blotting analyses of the interaction between STING and ICAD in HEK293T cells. E Immunoprecipitation and western blotting analyses of the interaction between STING and CAD upon overexpressing ICAD-NTD in HEK293T cells. F qPCR analyses of CCL5, CXCL10, and IFN-β mRNA in indicated cells at 24 h after irradiation. Error bars represent mean ± SD. P values were determined using two-tailed, unpaired t-test (B and F). *, P < 0.05; **, P < 0.01; ***, P < 0.001.
