Fig. 5: Irgm1-deficient inhibits the RANKL-induced NF-κB signaling.
From: Targeting Irgm1 to combat osteoporosis: suppressing ROS and restoring bone remodeling
A WB detected phosphorylated or total P65 and IκB in transfected RAW264.7 cells induced with 40 ng/ml RANKL for the indicated times. B–D Quantitative analysis of phosphorylated P65 (B), phosphorylated IκB (C), and total IκB (D) from (A) was normalized to GAPDH and presented as means ± SD (n = 3). ∗∗p < 0.01, and ∗∗∗p < 0.001 vs the indicated groups. E Immunofluorescence analysis of P65 level in different groups of RAW264.7 for 3 days. Scale bar in lower magnification = 50 μm, scale bar in higher magnification = 10 μm. F Quantitative results from (E) (n = 3). ∗p < 0.05 and ∗∗p < 0.01 vs the indicated groups.
