Fig. 2: Non-catalytic FLOS domain of SETD1A is required for GC cell growth. | Cell Death & Disease

Fig. 2: Non-catalytic FLOS domain of SETD1A is required for GC cell growth.

From: Non-catalytic role of SETD1A promotes gastric cancer cell proliferation through the E2F4–TAF6 axis in the cell cycle

Fig. 2

A SETD1A mRNA levels were measured using RT-qPCR every 2 days after Dox treatment in sgRNA-expressing iCas9-AGS cells. Results are presented as mean ± SD from three biological replicates. B SETD1A protein levels were analyzed using western blotting at the indicated time points. C Cell numbers were counted every 2 days in sgRNA-expressing iCas9-AGS cells after Dox treatment. Results are presented as mean ± SD from three biological replicates. Asterisks indicate statistical significance compared with sgEmpty at each time point. D Western blotting results of SETD1A and H3K4me1/2/3 in sgRNA-expressing iCas9-AGS cells after 6 days of Dox treatment. Representative images of three biological replicates are shown. E Heatmaps of H3K4me1/2/3 ChIP-seq signals in sgRNA-expressing iCas9-AGS cells after 6 days of Dox treatment. F ChIP-seq signal intensities of H3K4me1/2/3 in the indicated genomic regions. G Workflow of rescue experiment. H Schematic representation of SETD1A cDNA constructs. I Competitive growth assay of sgSETD1A-expressing and cDNA-transduced iCas9-AGS cells after Dox treatment. Results are presented as mean ± SD from three biological replicates.

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