Fig. 1: ZFP82 is highly methylated and downregulated in chemo-resistant esophageal cancer patient tissues. | Cell Death & Disease

Fig. 1: ZFP82 is highly methylated and downregulated in chemo-resistant esophageal cancer patient tissues.

From: Zinc Finger Protein 82 regulates p53 protein stability through histone deacetylase and enhances neo-adjuvant chemotherapy in esophageal cancer

Fig. 1

A Number of hypo-methylated and hyper-methylated gene in chemotherapy non-responder and pathologic complete responder esophageal tumor tissue samples through Infinium Human Methylation 850 Bead Chip. B Heatmap demonstrated ZFP82 is a highly methylated gene in non-responder tissues through Infinium Human Methylation 850 Bead Chip. C Volcano plot of hypo-methylated and hyper-methylated gene through Infinium Human Methylation 850 Bead Chip. D Differentially methylated probes within the ZFP82 promoter region were detected, with the green dot indicating a significantly hypermethylated region. E ZFP82 promoter methylation status by qMSP in NR and pCR esophageal tumor tissues (p < 0.05). F Representative image of MSP image in NR and pCR esophageal tumor tissues, M, methylated; U, unmethylated. G A CpG island in the ZFP82 promoter region was predicted using MethPrimer. H Methylated ZFP82 promoter alleles in three esophageal cancer cell lines (KYSE960/R, TE6/R and KYSE150/R) and four NPC tissue samples to confirm the result of MSP by Bisulfite genomic sequencing (BGS). I Differential mRNA expression of ZFP82 in chemotherapy non-responder and responder esophageal tumor tissue samples by semi-quantitative RT-PCR. NR chemotherapy non-responder, pCR pathologic complete responder.

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