Fig. 2: sNK cells secreted much higher levels of cytokines, chemokines, and growth factors compared to primary NK cells. | Cell Death & Disease

Fig. 2: sNK cells secreted much higher levels of cytokines, chemokines, and growth factors compared to primary NK cells.

From: Supercharged NK cells: a unique population of NK cells capable of differentiating stem cells and lysis of MHC class I high differentiated tumors

Fig. 2

OCs were generated as described in “Materials and methods” section. NK cells (1 × 106 cells/ml) from healthy individuals were treated with a combination of IL-2 (1000 U/ml) and anti-CD16 mAb (3 µg/ml) for 18 h before they were cultured with the healthy individuals’ OCs and sAJ2 at a ratio of 1:2:4 (OCs:NK:sAJ2). On day 15 of sNK cell cultures, another set of NK cells was isolated from the same healthy individuals and were treated with IL-2 (1000 U/ml). The supernatants were harvested from primary IL-2-treated NK cells and sNK cells to determine secretion levels of cytokines, chemokines, and growth factors using a multiplex assay (n = 4).

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