Fig. 5: Increased levels of IFN-γ secretion by sNK cells compared to primary activated NK cells. | Cell Death & Disease

Fig. 5: Increased levels of IFN-γ secretion by sNK cells compared to primary activated NK cells.

From: Supercharged NK cells: a unique population of NK cells capable of differentiating stem cells and lysis of MHC class I high differentiated tumors

Fig. 5

sNK cells were generated as described in Fig. 1. On day 15 of sNK cell cultures, another set of NK cells was isolated from the same healthy individuals and were treated with a combination of IL-2 (1000 U/ml) and anti-CD16 mAb (3 µg/ml) for 18 h. The supernatants were harvested from primary IL-2 and anti-CD16 mAbs-treated NK cells and sNK cells to determine IFN-γ secretion using a single ELISA (n = 4) (A). Fold change in IFN-γ was determined by using the formula: (left bar) IFN-γ of IL-2 + anti-CD16 mAbs-treated pNK cells/ IFN-γ of IL-2 + anti-CD16 mAbs-treated pNK cells, (right bar) IFN-γ of sNK cells/IFN-γ of IL-2 + anti-CD16 mAbs (n = 4) (B). The amounts of IFN-γ secretion shown in Fig. 5A were determined based on 1 × 106 cells (n = 4) (C). Fold change in IFN-γ was determined by using the formula: (left bar) IFN-γ per million cells of IL-2 + anti-CD16 mAbs-treated pNK cells/IFN-γ per million cells of IL-2+anti-CD16 mAbs-treated pNK cells, (right bar) IFN-γ per million cells of sNK cells/IFN-γ per million cells of IL-2 + anti-CD16 mAbs (n = 4) (D).

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