Fig. 7: Chemotherapeutic drugs induced higher killing in differentiated tumors.

Differentiation of OSCSCs (A) and MP2 (B) was conducted with an average total of 2000 pg and 5000 pg IFN-γ as described in Fig. 4. OSCSCs, OSCCs, primary NK cell-supernatant-treated OSCSCs, and sNK cell-supernatant-treated OSCSCs were treated with cisplatin (60 µg/mL) for 18–20 h, after which, the cells were stained with propidium iodide (PI) to determine percent cell death using flow cytometric analysis (A). MP2, PL12, primary NK cell-supernatant-treated MP2, and sNK cell-supernatant-treated MP2 were treated with paclitaxel (40 µg/mL) for 18–20 h, after which, the cells were stained with PI to determine percent cell death using flow cytometric analysis (B).