Fig. 4: CircMAP3K13 encodes a novel protein, MAP3K13-232aa. | Cell Death & Disease

Fig. 4: CircMAP3K13 encodes a novel protein, MAP3K13-232aa.

From: MAP3K13-232aa encoded by circMAP3K13 enhances cisplatin-induced pyroptosis by directly binding to IKKα in gastric adenocarcinoma

Fig. 4

A Mass spectrometry (IP-MS) following immunoprecipitation was conducted to identify the unique peptide of MAP3K13-232aa. B A commercial MAP3K13 antibody for MAP3K13 was used to detect the overexpressed MAP3K13-232aa and endogenous MAP3K13-232aa by immunoblotting. C MAP3K13-232aa expression level was analyzed in nine pairs of GC and paired adjacent normal tissues by western blot. N normal tissue, T tumor tissue. D Immunoblot analysis of MAP3K13-232aa across various cell lines, including GC cell lines (NCI-N87, MKN-45, HGC-27, AGS, MKN-28), a normal gastric epithelial cell line (GES-1), and 293 T cells. GAPDH served as the loading control. E Schematic of three siRNAs targeting circMAP3K13. F qPCR results of circMAP3K13 expression after transfection with si-1, si-2, and si-3. G Western blot analysis of MAP3K13-232aa protein as well as full-length MAP3K13 after circMAP3K13 siRNA transfection. GAPDH served as the loading control. Unless otherwise specified, statistical significance was assessed using a two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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