Fig. 5: circMAP3K13 suppresses the proliferation and migration of GC cells. | Cell Death & Disease

Fig. 5: circMAP3K13 suppresses the proliferation and migration of GC cells.

From: MAP3K13-232aa encoded by circMAP3K13 enhances cisplatin-induced pyroptosis by directly binding to IKKα in gastric adenocarcinoma

Fig. 5

A Cell proliferation was determined by using EdU staining (red) after overexpression of MAP3K13-232aa in AGS cells and B after silencing circMAP3K13 with siRNAs transfection in HGC-27 cells. The nuclei are counterstained with DAPI (blue). Scale bar = 10 μm. C Quantification of EdU-positive cells from (A, B). D Wound healing assay was performed after circMAP3K13 and MAP3K13-232aa overexpression. Scale bar = 100 μm. E Wound healing assay was performed after knockdown of circMAP3K13 in HGC-27 cells. Scale bar = 200 μm. F Quantification of wound healing assays is shown. G Transwell assay was conducted after overexpressing MAP3K13-232aa in AGS cells. Scale bar = 100 μm. H Transwell assay was conducted after transfection of circMAP3K13-targeting siRNAs in HGC-27 cells. Scale bar = 100 μm. I Quantification of Transwell invasion assay results is shown. Unless otherwise specified, statistical significance was assessed using a two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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