Fig. 7: MAP3K13-232aa enhances cisplatin-induced pyroptosis through activating the NF-κB signaling pathway.

A Western blot analysis of AGS cells transfected with circMAP3K13 overexpression plasmids. Cells were treated with 20 ng/mL TNFα for 5 min prior to protein collection. NF-κB signaling pathway-related proteins were detected by western blot. B Detection of NF-κB downstream target proteins in AGS cells transfected with circMAP3K13 overexpression plasmids. C AGS cells transfected with either an empty vector or circMAP3K13 overexpression plasmid circMAP3K13 groups were treated with LPS (2 μg/mL) for 4 h and nigericin (20 μM) for 1 h. Red arrows indicate protrusive vesicles characteristic of pyroptosis. D LDH levels in the culture supernatant were measured in AGS cells under the same treatment conditions to evaluate the extent of pyroptosis. E NLRP3 mRNA expression was assessed in AGS cells after induction of pyroptosis. F Western blot analysis was used to detect NLRP3 protein expression following pyroptosis induction after circMAP3K13 overexpression and knockdown. Western blot analysis of pyroptosis-related proteins in MKN-45 (G) or N87 (H) cells, which were treated with cisplatin (1 μg/mL) for 48 h. I Rescue assay showing the effect of MAP3K13-232aa overexpression following circMAP3K13 knockdown on pyroptosis-associated protein expression. Unless otherwise specified, statistical analysis was performed using a two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.