Fig. 3: Honokiol causes a decrease of tumor mass and metastases in vivo.
From: Honokiol blocks tumor development and metastasis through mitochondrion-targeted effects
A CTR and IF1 KO HeLa cells, are injected into the yolk of zebrafish aged 2 days post fertilization (dpf). Fish are treated with 1‰ DMSO, 0.1 or 1 µM HK. Images (i) of fish (DMSO and 1 µM HK) at 6 dpf are shown. Red fluorescence indicates tumor masses derived from HeLa cells. Scale bar, 1 000 μm. Histogram (ii) represents the mean of fluorescence at 6 dpf of HeLa cells. Fluorescence indicates tumor mass development in fish treated with 1‰ DMSO, 0.1 or 1 µM HK. Data are mean ± SEM of six independent experiments. **p = 0.0081, ****p ≤ 0.0001, Student’s t test. B Histograms represent the % of fish injected with CTR (i) and IF1 KO (ii) HeLa cells showing tumor in place, or initial and full metastasis. C Total number of CTR or IF1 KO HeLa cells forming primary tumor (i) or metastases (ii) in zebrafish (6 dpf) injected as above. Data are mean ± SEM of six independent experiments, *p ≤ 0.05, **p = 0.0072, ***p ≤ 0.001, ****p ≤ 0.0001, Student’s t test.
