Fig. 1: TRPM8 is overexpressed in metastatic melanoma cells and localizes to both the plasma membrane and endoplasmic reticulum. TRPM8 modulators reduce melanoma cell viability.
From: Rewiring melanoma cell fate: TRPM8 modulators trigger apoptosis and boost NK cell cytotoxicity
A TRPM8 gene expression in melanoma tissues (red, T; n = 461) vs. normal skin (green, N; n = 558) from The Genomic Data Commons (GDC) Cancer Genome Atlas (TCGA) database. Data are presented as log2 TPM; p < 0.001 by unpaired t-test. B Representative westen blot showing TRPM8 protein expression in the indicated cell lines. Tubulin was used as a loading control. Immunofluorescence analysis of TRPM8 localization in WM266-4 (C) and AMM16 cells (D). Cells were stained with anti-TRPM8 antibody (blu), Endoplasmic reticulum (ER)-tracker (green) and Plasma Membrane-tracker (red). Merged images indicate TRPM8 distribution in both plasma membrane and ER compartments. Scale bar: 10 µm. Viability of AMM16 (E) and WM266-4 (F) cells untreated or treated with compounds 3, 5, 4, 6 and 9 at the concentrations indicated in the legends above. Absorbance values from WST-1 assays after 24, 48 and 72 h are reported. Data are expressed as mean ± standard deviations (SDs) from n independent experiments. *p < 0.05 for the indicated time points vs. the corresponding untreated control.
