Fig. 4: ΔNp63α directly transactivates gene expression of serine synthesis enzymes.
From: ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC
A HCC95 cells stably expressing a control shRNA (shC) or two different shRNAs specific for p63 were subjected to QPCR analysis. B NCI-H1703 or HCC95 cells stably expressing a control shRNA (shC) or two different shRNAs specific for p63 were subjected to immunoblot analysis. C p63 chromatin immunoprecipitation (ChIP) sequencing data from human keratinocytes (GEO accession number GSE32061) identifies putative p63-binding sites within the promoter or enhancer regions of PHGDH, PSAT1, PSPH, and SLC1A4 genes. D HEK-293T cells were co-transfected with reporter plasmids (PHGDH-Gluc, PSAT1-Gluc, PSPH-Gluc, or SLC1A4) and ΔNp63α (WT or R304W) expression plasmid. Gluc and SEAP activities in the media were measured at 48 h post-transfection. E ChIP assays using ΔNp63 antibody or normal rabbit IgG were performed in HCC95 cells. *p < 0.05; **p < 0.01; NS not significant.
