Fig. 7: Steroids and DAG analogues did not inhibit SubAB-induced apoptosis in mouse intestinal organoids and MEF cells

a Mouse intestinal organoids were incubated for 30 min on ice (Top panel) or for 2 h at 37 °C (bottom panel) with cy3-labeled SubAB and then fixed as described in Materials and methods. b, c, e, and f shows high-magnification views of the white rectangular areas in a and d, respectively. Experiments were repeated three times with similar results. b The organoids were treated for 12 h with or without Dx (3.3 μg/ml), Bry (10 nM) or I3AG (2.5 nM), and then incubated for 24 h with mt or wt SubAB (0.2 μg/ml). Cell lysates were subjected to immunoblotting with the indicated antibodies. Experiments were repeated three times with similar results. c MEF cells were treated for 12 h with or without Dx (3.3 μg/ml), Bry (10 nM) or I3AG (2.5 nM), and then incubated for 24 h with mt or wt SubAB (0.2 μg/ml). Cell lysates were subjected to immunoblotting with the indicated antibodies. Experiments were repeated three times with similar results