Fig. 6: Testicular distribution and molecular function of ZCCHC13. | Cell Death Discovery

Fig. 6: Testicular distribution and molecular function of ZCCHC13.

From: Novel biomarker ZCCHC13 revealed by integrating DNA methylation and mRNA expression data in non-obstructive azoospermia

Fig. 6

a Immunoblotting analysis were employed to assess the expression level of ZCCHC13 in GC-1 and TM4 cells. GAPDH serves as a loading control. b Immunohistochemistry demonstrating ZCCHC13 protein presented in germ cells from testis biopsy from man with OA controls (normal spermatogenesis), and had significantly decreased expressions of ZCCHC13 protein in man with NOA at low power 100× and high power 400×. c TM4 cells and d GC-1 cells show that the expression levels of c-MYC, p-AKT, and p-ERK were remarkably increased following ZCCHC13 overexpression. GAPDH served as a loading control

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