Fig. 7: Increased expression of ZCCHC13 in response to 5-Aza treatment. | Cell Death Discovery

Fig. 7: Increased expression of ZCCHC13 in response to 5-Aza treatment.

From: Novel biomarker ZCCHC13 revealed by integrating DNA methylation and mRNA expression data in non-obstructive azoospermia

Fig. 7

a MSP analyses showed that all the five different concentrations of 5-Aza can attenuate the DNA methylation within the promoter of ZCCHC13 in a dose-dependent manner in GC-1 cells. U unmethylated MSP amplicons; M methylated MSP amplicons. b RT-PCR analysis of ZCCHC13 expression in 5-Aza-treated GC-1 cells. c Quantitative PCR analysis in terms of ZCCHC13 expression levels were normalized to those of the internal control GAPDH. Values are the mean ± S.D. (error bars) of at least three determinations. Significant differences (*p < 0.05, **p < 0.01) between the control (DMSO) and experimental groups are marked with asterisks. d GC-1 cells were treated with various doses of 5-Aza for 48 h. The protein levels of ZCCHC13, p-AKT1, AKT1, p-ERK1/2, ERK, and CDK4 measured by Western blotting analysis

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