Fig. 8: 5-Aza suppressed the GC-1 cells growth.

a Flow cytometric analysis of cell cycle in GC-1 cells treated with 5-Aza at the indicated doses for 24 h. Cells were analyzed by flow cytometry after staining with PI. b Quantization of flow cytometry data according to cell cycle phase for the groups indicated. c 5-Aza significantly inhibited cell proliferation in a dose-dependent and time-dependent manner. The cell viability was determined by MTS assay (n = 3). Data represent the mean ± SD. Significant differences (*p < 0.05, **p < 0.01) between the control (DMSO) and experimental groups are marked with asterisks