Fig. 2: Drp1 knockdown in human iPSCs induces cardiac mesoderm differentiation. | Cell Death Discovery

Fig. 2: Drp1 knockdown in human iPSCs induces cardiac mesoderm differentiation.

From: Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells

Fig. 2

a Western blotting analysis of Drp1 expression (n = 4 independent experiments) and b RT-qPCR analysis of mitochondrial fusion and fission gene expression (n = 7–11 independent experiments) in iPS-Foreskin-2 cells treated with scrambled (control) or DRP1 siRNA. c Morphology of mitochondria and percentage of Oct3/4-positive cells with different mitochondrial morphologies (n = 4 independent experiment). d–f mRNA expression of cardiac mesoderm transcription factors (d), pluripotency genes (e) and cell proliferation genes (f) in iPS-Foreskin-2 cells treated with scrambled (control) or Drp1 siRNA (n = 8–11 independent experiments). Data are shown as mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.0001 by unpaired Student’s t-test

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