Fig. 3: Loss of function of Pont produces JNK-mediated cell death phenotype in adults.

Light images of Drosophila thoraxes (a–h) are shown. pnr-Gal4 was used as a control (a), or to drive the expression of pont-IR (b–d, f–h) or UAS-Pont (c). pnr>pont-IR induced small scutellum phenotype (b, i), was suppressed by expression of Pont (c, i) and enhanced in puc heterozygous mutant background (d, i), while puc heterozygous mutant alone showed no effect on the scutellum (e, i). pnr>pont-IR-induced small scutellum phenotype (b, i) was suppressed by in bsk heterozygous mutant (f, g, i) and fos heterozygous mutant (h, i) background. i Statistical analysis of the scutellum size in (a–h). Error bars means ± SEM, ***P ≤ 0.001, **P ≤ 0.01. Genotypes: pnr-Gal4/+ (a); UAS-pont-IR #2/+; pnr-Gal4/+ (b); UAS-pont-IR #2/UAS-Pont; pnr-Gal4/ UAS-Pont (c); UAS-pont-IR #2/+; pnr-Gal4/puc^E69 (d); pnr-Gal4/puc^E69 (e); UAS-pont-IR #2/+; pnr-Gal4/bsk^1/+(f); UAS-pont-IR #2/+; pnr-Gal4/bsk^2/+(g); UAS-pont-IR #2/+; pnr-Gal4/fos^1/+(h)