Fig. 4: Loss of function of Pont triggers JNK phosphorylation.
From: Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
Fluorescent images of the immunostaining for JNK phosphorylation (pJNK) (a–a”’, b–b”’, c–c”’) are shown. en-Gal4 was used as a control (a–a”’), or to drive the expression of pont-IR (b–b”’, c–c”’) or UAS-Puc (c–c”’). Compared with the en-Gal4 alone (a–a”’), en>pont-IR induced strong JNK phosphorylation (b’, b”, b”’, indicated by white arrow in b’ and red arrow in b”), which was abolished by expression of Puc (c–c”’). Scale bar, 200 μm. Genotypes: en-Gal4 UAS-GFP/+ (a–a”’); en-Gal4 UAS-GFP /UAS-pont-IR #2 (b–b”’); en-Gal4 UAS-GFP /UAS-pont-IR #2; UAS-Puc/+ (c–c”’)
