Fig. 5: Expression of Pont inhibits Egr-induced cell death in the developing eye.

Light images of adult eyes (a–d) and fluorescent images of acridine orange staining (e–h) are shown. GMR-Gal4 was used as a control (a, e) or to drive the expression of Egr (b–d, f–h) or UAS-Pont (c, g), UAS-LacZ (d, h). GMR>Egr-induced small eye phenotype (b) and cell death (f) were suppressed by expression of Pont (c, g) not by expression of LacZ (d, h). i Statistical analysis of the eye size in (a–d). Error bars means ± SEM, ***P ≤ 0.001, n.s. not significant. j Statistical analysis of acridine orange-positive cells in (e–h). Error bars means ± SEM, ***P ≤ 0.001, n.s. not significant. Scale bar, 100 μm. Genotypes: GMR-Gal4/+ (a, e); UAS-Egr^S/+; GMR-Gal4/+ (b, f); UAS-Egr^S/UAS-Pont; GMR-Gal4/ UAS-Pont (c, g); UAS-Egr^S/+; GMR-Gal4/ UAS-LacZ (d, h)