Fig. 6: Expression of Pont inhibits Egr-induced cell death phenotype in the thorax and wing.
From: Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
Light images of Drosophila adult thoraxes (a–c), Drosophila wings (d–f) and X-gal staining of third-instar larva wing disc (g–i) are shown. ptc-Gal4 was used as a control (a, d, g), or to drive the expression of Egr (b, c, e, f, h, i) or UAS-Pont (c, f, i), or UAS-LacZ (b, e). The lower panels are magnification of the boxed area in the upper panels. Compared with the ptc-Gal4 control (a, d, g), ptc>Egr-induced small scutellum (b), loss of anterior cross vein (ACV) phenotype (e) and puc activation (h) were suppressed by expression of Pont (c, f, i). j Statistical analysis of the scutellum size in (a–c). Error bars means ± SEM, ***P ≤ 0.001. k Statistical analysis of the presence of ACV in (d–f). Error bars means ± SEM, ***P ≤ 0.001. Genotypes: ptc-Gal4/+; pucE69/+ (a, d, g); ptc-Gal4 UAS-EgrW/+; pucE69/UAS-LacZ (b, e); ptc-Gal4 UAS-EgrW/+; pucE69/+ (h); ptc-Gal4 UAS-EgrW /UAS-Pont; pucE69/ UAS-Pont (c, f, i)
