Fig. 7: Expression of Pont inhibits Hep-induced cell death.

Light images of Drosophila adult eyes (a–d), thoraxes (f–i) and Drosophila wings (k–n) are shown. GMR-Gal4 was used as a control (a) or to drive the expression of Hep (b–d) or UAS-Pont (c) or UAS-LacZ (d). GMR>Hep-induced cell death phenotype (b) was suppressed by expression of Pont (c) and not by expression of LacZ (d). ptc-Gal4 was used as a control (f, k) or to drive the expression of Hep (g–i, l–n) or UAS-Pont (h, m) or UAS-LacZ (i, n). ptc>Hep-induced small scutellum phenotype (g) and loss of ACV phenotype (l) were suppressed by expression of Pont (h, m) and not by expression of LacZ (i, n). The lower panels are the magnification of boxed area in the upper panels. e Statistical analysis of the eye size in (a–d). Error bars means ± SEM, ***P ≤ 0.001, **P ≤ 0.01, n.s. not significant. j Statistical analysis of the scutellum size in (f–i). Error bars means ± SEM, ***P ≤ 0.001, n.s. not significant. o Statistical analysis of the presence of ACV in (k–n). Error bars means ± SEM, ***P ≤ 0.001, n.s. not significant. Genotypes: GMR-Gal4/+ (a); GMR-Gal4 UAS-Hep^CA/+ (b); UAS-Pont/+; GMR-Gal4 UAS-Hep^CA/UAS-Pont (c); GMR-Gal4 UAS-Hep^CA/UAS-LacZ (d); ptc-Gal4/+; puc^E69/+ (f, k); ptc-Gal4/UAS-Hep^WT; puc^E69/+ (g, l); ptc-Gal4 UAS-Hep^WT/UAS-Pont; UAS-Pont/ puc^E69 (h, m); ptc-Gal4 UAS-Hep^WT/+; UAS-LacZ/ puc^E69 (i, n)