Fig. 2: Histological analyses of EAE-induced monkeys.

A LFB/eosin staining was performed to assess demyelination in the brain (a) and spinal cord (b) of EAE-induced monkeys. Immunostaining for MBP and GFAP indicates astrocytes activation surrounding lesions in the brain (c) and spinal cord (d) in the EAE-induced monkey C1 but not the normal control C8. B Immunostaining for αSMA, MBP, and GFAP shows (a) damages of blood–brain barrier in a lesioned area (lower panels) compared with a normal area (upper panels) in the brain of the EAE-induced monkey C5. Immunostaining for CD3 and oligodendrocytes shows (b) lymphocyte infiltration from a blood vessel to the brain of the EAE-induced monkey C1. Scale bars, 50 μm