Fig. 1: YL064 selectively induces myeloma cell death.
From: YL064 directly inhibits STAT3 activity to induce apoptosis of multiple myeloma cells
a Chemical structure of YL064. b U266 and MM1.S cells were treated with the indicated concentrations of YL064 for 24 h and apoptotic cells were evaluated by Annexin V/PI double-staining assay. **p < 0.01, compared with control annexin V+/PI– cells, ##p < 0.01, compared with control annexin V+/PI+ cells. c U266 and MM1.S cells were untreated or treated with indicated concentrations of YL064 for 24 h. The indicated proteins were detected by western blot. d Primary CD138+ MM cells were treated with YL064 (20 μM) for 24 h and CD138+ apoptotic cells were evaluated by Annexin V/PI double-staining assay. **p < 0.01, compared with control annexin V+/PI– cells, #p < 0.05, ##p < 0.01, compared with control annexin V+/PI+ cells. e Primary PBMCs cells from healthy donors were treated with the indicated concentrations of YL064 (40 μM) for 24 h and cell viability was evaluated by Trypan blue exclusion assay
