Fig. 4: YL064 inhibits HS-5 co-culture-induced activation of STAT3 in myeloma cells. | Cell Death Discovery

Fig. 4: YL064 inhibits HS-5 co-culture-induced activation of STAT3 in myeloma cells.

From: YL064 directly inhibits STAT3 activity to induce apoptosis of multiple myeloma cells

Fig. 4

a HS-5, U266, and MM1.S cells were treated with the indicated concentrations of YL064 for 24 h. Cell viability was determined by Trypan blue assay. Values represented as graphs are the mean of three independent experiments with the standard deviation. b-d U266 and MM1.S cells were seeded on an established HS-5 stromal layer for 24 h, then the cells were treated with YL064 (20 μM) for additional 24 h. Subsequently, cells were separated from the HS-5 stromal layer, and apoptotic cells were examined by Annexin V/PI double-staining (b). **p < 0.01, compared with control annexin V+/PI– cells, ##p < 0.01, compared with control annexin V+/PI+ cells. The indicated proteins were examined by western blot (c, d)

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