Fig. 1: Exposure of w¹¹¹⁸ flies to rotenone-induces shortened lifespan and severe locomotor deficits in a dose- and age-dependent manner.

a Schematic representation of rotenone treatment in the 1-day old flies for survival curve assay. b Survival curve of 1-day old flies exposed to varying concentration of rotenone (50 , 100 , and 200 μM). Fly viability was scored over a period of 40-days using a minimum of 100 flies per treatment. The statistical significance was calculated as log-rank using Mantel–Cox test. c–e Negative geotaxis assay of 1-day old flies exposed to rotenone at concentrations indicated above for 1-day (c), 10-days (d) and 20-days (e). f Schematic representation of rotenone treatment in the 1-day, 10-day, and 20-day old flies for survival curve assay. g Age-dependent survival of flies exposed to 100 µM of rotenone at 1-day, 10-day, and 20-day following eclosion. Fly viability was scored up to 40-days, post-eclosion using a minimum of 100 flies per treatment. h Negative geotaxis assay of 20-day old flies exposed to 200 μM rotenone for 3-days. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to control flies