Fig. 3: Early induction of dSarm is necessary and sufficient for rotenone-induced neurotoxicity.

a Schematic representation of rotenone treatment of 1-day old flies for 10-days followed by withdrawal and further experiments were performed at the time points indicated. b Survival curve of control adult flies and flies exposed for 10-days to 200 μM of rotenone and subsequent transfer of these flies to normal media. Fly viability was scored over a period of 40-days using a minimum of 100 flies per treatment. c Negative geotaxis assay of adult flies exposed to rotenone as indicated above for 10-days and experiment performed at day-20 post-exposure. d dSarm expression was analyzed at day 20 of flies treated above and results compared to age-matched untreated control flies. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to control flies