Fig. 7: Sarm1 is required for rotenone-induced toxicity in SH-SY5Y cells. | Cell Death Discovery

Fig. 7: Sarm1 is required for rotenone-induced toxicity in SH-SY5Y cells.

From: Sarm1 induction and accompanying inflammatory response mediates age-dependent susceptibility to rotenone-induced neurotoxicity

Fig. 7

a Immunofluorescence analysis of SH-SY5Y cells treated with 5 μM of rotenone for 24 h (bottom panel) and compared to untreated control cells (top panel). DAPI staining of both the treated and untreated samples are shown in the right-hand panel. b, c Real-time PCR analysis of cells treated with 2.5 or 5 μM of rotenone. Both TNFa (b) and Sarm1 (c) genes were analyzed following 24 h post-treatment. d, e Real-time PCR analysis of cells as treated with rotenone (5 μM) in the presence or absence of resveratrol (5 or 20 μM as indicated). Both TNFa (d) and Sarm1 (e) genes were analyzed following 6 h post-treatment. f, g Real-time PCR analysis of cells treated with rotenone (5 μM) in the presence or absence of neutralizing antibody against TNFα (5 mg/ml). The expression of both TNFa (f) and Sarm1 (g) were analyzed following 24 h post-treatment. h MTT assay of cells treated with rotenone (5 μM) in the presence or absence of neutralizing antibody against TNFα (5 mg/ml) for 24 h. i SH-SY5Y cells were treated with 25 nM of Sarm1 siRNA or control (nontargeting) siRNA for 24 h followed by 5 μM of rotenone treatment for 24 h. MTT assay of cells treated with rotenone in the presence or absence of siRNAs was performed. j Analysis of SARM1 protein levels in the whole cell extracts of cells transfected with Sarm1 or control siRNA in the presence or absence of rotenone (5 μM). β-actin was used as a loading control. Results are representative of at least three independent experiments. *p < 0.05, **p < 0.01, and ***p<0.001 compared to control flies

Back to article page