Fig. 4: Overexpression of Mcl1 protects against apoptotic stress.
From: Survival of midbrain dopamine neurons depends on the Bcl2 factor Mcl1
a Immunofluorescent staining of cells transfected with empty vector or plasmid encoding for Mcl1. Cell were co-transfected with GFP as a marker for transfected cells and subsequently treated with etoposide for 8 h before treatment with PI. Scale bar represents 50 µm. b Left: blow up of representative example of GFP positive cell and dual GFP and PI positive cell. Scale bar represents 5 µm. Right: quantification of results seen in a. The amount of cleaved caspase 3 positive cells was determined relative to total GFP positive cells. Significance was determined with a Student’s t-test, p value: **p < 0.01 (n = 3)
