Table 2 Primer sequences used in the qRT/PCR analyses
Primers | Nucleotide sequence (5′–3′) | T annealing (°C) | NCBI reference sequence | Efficiency (%) |
|---|---|---|---|---|
GAPDH Fw GAPDH Rev | GAAGGTGAAGGTCGGAGT TGGCAACAATATCCACTTTACCA | 60 | NM_17851.1 | 99.0 |
HPRT1 Fw HPRT1 Rev | CCTGGCGTCGTCATTAGTGA TCTCGAGCAAGACGTTCAGT | 60 | NM_000194.2 | 97.8 |
Osteonectin Fw Osteonectin Rev | CCTGGATCTTCTTTCTCCTTTGC ATCAGGCAGGGCTTCTTGCT | 60 | NM_001309443.1 | 97.9 |
Osteopontin Fw Osteopontin Rev | CGAGACCTGACATCCAGTACC GATGGCCTTGTATGCACCATTC | 62 | NM_001251830.1 | 96.2 |
Runx2 Fw Runx2 Rev | CATTTCAGATGATGACACTGCC GGATGAAATGCTTGGGAACTG | 62 | NM_001024630.3 | 95.2 |
ALP Fw ALP Rev | GGCAACTCTATCTTTGGTCTG GTTGTTGTGAGCATAGTCCA | 60 | NM_000478.4 | 95.0 |
BAX Fw BAX Rev | GCCGTGGACACAGACTCC AAGTAGAAAAGGGCGAAACC | 60 | NM_001291428.1 | 91.2 |
BCL-2 Fw BCL-2 Rev | TCATGTGTGTGGAGAGCGTCAA CAGCCAGGAGAAATCAAACAGAGG | 60 | NM_000633.2 | 88.9 |
