Fig. 1: AJ-5 shows potent and selective cytotoxicity against aRMS and eRMS cells. | Cell Death Discovery

Fig. 1: AJ-5 shows potent and selective cytotoxicity against aRMS and eRMS cells.

From: The palladacycle complex AJ-5 induces apoptotic cell death while reducing autophagic flux in rhabdomyosarcoma cells

Fig. 1

a MTT cell viability assays of aRMS cell lines, RH30 and AX-OH-1, and eRMS cell lines, RD and FL-OH-1, treated with a range of AJ-5 concentrations (0.1–1.0 µM) or vehicle for 48 h. Graphs show mean cell viability as a percentage of vehicle control ± SEM for each concentration of AJ-5 determined from three independent experiments performed in quadruplicate. A curve was fitted to determine the IC50 concentration of AJ-5 for each cell line. b Representative light microscopy images (×200; EVOS XL AMEX1000 Core Imaging System) of aRMS and eRMS cell lines treated with their respective IC50 concentrations of AJ-5 or vehicle for 24 and 48 h. c MTT cell viability assays of non-malignant human fibroblast cell lines, FG0 and DMB, mouse myoblast cell line, C2C12, and mesenchymal stem cell line, A10021501, treated with AJ-5 and IC50 concentrations determined as described in a above. Selectivity indices (SIs) were determined for each RMS cell line by dividing the IC50 of each non-malignant cell line by the IC50 of each RMS cell line

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