Fig. 3: Sirt6 participates in ox-LDL-decreased ECs autophagy.

a ox-LDL-decreased ECs autophagosome formation. Green, GFP; red, RFP-labeled LC3; yellow, green, and yellow double-labeled autophagosome. Scale bar: 100 μm. b ox-LDL inhibited Sirt6 expression and decreased autophagic biomarkers expression with or without chloroquine. Chloro, Chloroquine. c Adv Sirt6 or LV Sirt6 significantly changed Sirt6 mRNA expression. d Sirt6 KI or Sirt6 KD effectively regulated ECs autophagic biomarkers expression. e Sirt6 KI or Sirt6 KD effectively regulated ECs autophagosome formation. N = 3. Scale bar: 100 μm