Fig. 2: Muscarinic receptor subtypes expression. | Cell Death Discovery

Fig. 2: Muscarinic receptor subtypes expression.

From: M2 receptors activation modulates cell growth, migration and differentiation of rat Schwann-like adipose-derived stem cells

Fig. 2

a dASCs, as well as undifferentiated ASCs express all muscarinic receptors transcripts, except M5. GAPDH was used as housekeeping gene and brain lysates were used as positive controls. b Western blot showed M2 muscarinic receptor expression in ASCs and dASCs. β-actin was used as protein reference. c Densitometric analysis did not show any particular variation of M2 protein levels after SCs phenotype induction. Data are represented as mean ± SEM of three independent experiments and normalised against ASCs as control group. d Levels of cyclic AMP (cAMP) in dASCs. cAMP levels decreased after 2 h of treatment with APE 100 µM compared to untreated controls (**p < 0.01)

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