Fig. 3: Inhibition of RIDD mitigated 24S-OHC-induced cell death in SH-SY5Y cells. | Cell Death Discovery

Fig. 3: Inhibition of RIDD mitigated 24S-OHC-induced cell death in SH-SY5Y cells.

From: 24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death

Fig. 3

a SH-SY5Y cells were pretreated with 5 μM F12511 for 15 min or with 10 μM 4μ8 C for 1 h and then exposed to 50 μM 24S-OHC for 6 h. The mRNA levels of RIDD target genes (ANGPTL3, BLOS1, COL6, PDGFRB, and SCARA3) were quantified by real-time PCR. Results are shown as normalized to levels measured for RPL32. *P < 0.05, **P < 0.01, when compared with cells treated with vehicle or among 24S-OHC-treated groups. b Cells were pretreated with 2.5–7.5 μM MKC-3946 for 1 h then exposed to 50 μM 24S-OHC for 24 h. Cell viability was measured by LDH assay. **P < 0.01, when compared with cells treated with 24S-OHC alone

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