Fig. 4: miR-16/14-3-3η regulated CSCs properties and sorafenib resistance.

a, b HuH7SR cells were transfected by scrambled, miR-16 mimic, or miR-16 mimic plus 14-3-3η plasmid. a qRT-PCR analysis of the expressions of CD133 and EpCAM mRNAs. b Flow cytometry analysis in triplicate of the ratio of CD133+-EpCAM+ and SP cells. c After HuH7SR cells were pre-transfected as described above, they were treated by sorafenib. Cell viabilities were analyzed in triplicate by CCK-8 solution