Fig. 4: Pristimerin induces JNK activation and ROS generation. | Cell Death Discovery

Fig. 4: Pristimerin induces JNK activation and ROS generation.

From: Pristimerin induces apoptosis and autophagy via activation of ROS/ASK1/JNK pathway in human breast cancer in vitro and in vivo

Fig. 4

a, b MDA-MB-231 and MDA-MB-468 cells were treated with various concentrations of pristimerin for the indicated length of time. c, d The cells were preincubated with 10 μM JNK inhibitor (SP600125), ERK inhibitor (PD98059) or p38 inhibitor (SB203580) for 2 h before a 24 h pristimerin treatment. e, f MDA-MB-231 and MDA-MB-468 cells were pretreated with or without NAC (5 mM) for 1 h before a 6 h exposure to pristimerin (0.4 μM) and then treated with DCFH-DA for 30 min. Intracellular ROS generation was measured by fluorescence microscopy (×100). Bar: 100 μm. The results of the quantitative analysis of ROS are shown as histograms. (g) The cells were preincubated with NAC for 2 h and then treated with pristimerin for 24 h. The levels of p-JNK and JNK were analyzed by Western blotting. Results from three independent experiments are presented. *P < 0.05, **P < 0.01 and ***P < 0.001 versus control, #P < 0.05, ##P < 0.01 and ###P < 0.001 versus pristimerin treatment

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