Fig. 6: SERCA2b expression correlates with PERP protein stabilization and apoptosis induction. | Cell Death Discovery

Fig. 6: SERCA2b expression correlates with PERP protein stabilization and apoptosis induction.

From: ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER–plasma membrane junctions

Fig. 6

a mCherry (24 h) or mCherry-SERCA2b were expressed in Mel202 cells and PERP protein levels were detected by Western blot. b Graph shows PERP mRNA and protein levels normalized to the level of GAPDH in Mel202 cells expressing mCherry and mCherry-SERCA2b. Student’s t-test, n = 3, 48 h PT: p = 0.005**; 72 h PT: p = 0.0017**. c The percentage of apoptotic Mel202 cells expressing eGFP and eGFP-SERCA2b was measured at 24 and 48 h post-transfection determined by flow cytometry using Alexa Fluor 647 annexin V. Student’s t-test, n = 3, 24 h PT: p = 0.4924 ns, 48 h PT: p = 0.0048**. d Super-resolution images of HeLa BAC Venus-PERP cells expressing mCherry-SERCA2b for 24 and 48 h; scale bars: 20 µm

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