Fig. 4: miR-708 targets SESN3 in control PC and NEPC cells. | Cell Death Discovery

Fig. 4: miR-708 targets SESN3 in control PC and NEPC cells.

From: Targeting Wnt/EZH2/microRNA-708 signaling pathway inhibits neuroendocrine differentiation in prostate cancer

Fig. 4

a Schematic graph of 3′UTR of SESN3 with putative binding sites of miR-708. The chromosome locations of sites 1–4 are chr11: 94903044–94903054, chr11: 94902521–94902545, chr11: 94899816–94899839, and chr11: 94903269–94903285, respectively. b Luciferase activity in HEK293T cells co-transfected with luciferase reporter plasmids containing four different predicted miR-708-binding sites on the SESN3 3′-UTR, respectively, together with miR-C or miR-708. Two-sided independent Student’s t-tests were used to compare expression difference between two groups. *P < 0.05, **P < 0.01. (c) Western blotting analysis of SESN3 in LNCaP control PC cells and NEPC cells transfected with miR-C or miR-708. d pFOXO1 levels in SESN3-silenced LNCaP cells. e Schematic diagram depicting effect of miR-708 on FOXO1 phosphorylation via SESN3 in control and NEPC cells. Error bars represent the standard deviation of biological triplicates

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