Fig. 6: Effects of CDK1 and Wnt signaling on EZH2 expression in NEPC cells.

a Effect of CDK1 activation on EZH2 expression in control and NEPC cells. C4–2 and LNCaP cells and their corresponding NEPC cells were treated with docetaxel (DTX; 10 nM) for 24 h followed with or without the CDK1 inhibitor RO3306 (RO) 24 h. The cells were then subjected to western blotting analysis. b Effects of WNT signaling activation and deactivation on NEPC markers expression. C4–2 and LNCaP cells were treated with GSK-3 inhibitor IX (BIO, 2.5 μM) for 1 day following 1 day culture in control or NEPC-inducing medium. TCF4-knockdown C4–2 and LNCaP cells were cultured in NEPC-inducing medium for 6 days. The cells were then lysed for qPCR analysis. ****P < 0.0001. c Western blotting analysis of LNCaP control and NEPC-induced cells. d Schematic diagram depicting the upstream and downstream signaling of NEPC. Error bars represent the standard deviation of biological triplicates