Fig. 3: Pancreatic β cells are more susceptible to metformin than MEF cells. | Cell Death Discovery

Fig. 3: Pancreatic β cells are more susceptible to metformin than MEF cells.

From: Essential role of glucokinase in the protection of pancreatic β cells to the glucose energetic status

Fig. 3

a Representative western blot analysis (n = 3) of MIN6 and MEF cells challenged to a metformin dose-response (1–10 mM in the presence of 25 mM of glucose). In the plot is represented the densitometric analysis of the image. b Representative phase contrast images comparing pancreatic β cells such as MIN6 or INS1E cells with non-pancreatic β cells such as MEF or HEK in response to different doses of metformin (0, 5–1–2 mM). Scale bar, 40 µm. c Representative western blot analysis showing the cleaved caspase-3 protein levels in a time-course of metformin at 2 mM in MIN6. d Representative western blot analysis showing the cleaved caspase-3 protein levels in a time-course of metformin at 2 mM in MEF cells. e Representative quantification of violet crystal analysis of the number of viable cells after treatment with dose-response of metformin for 48 h in MIN6. The percentage of change over control represents the mean ± SD. *P < 0.05. f Flow cytometry analysis of the percentage of apoptotic MIN6 cells after a dose-response of metformin treatment using Annexin-V and PI incorporation. PI stands for propidium iodide

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