Fig. 1: The D2 background did not alter protection conferred by Ddit3 deletion after axonal injury. | Cell Death Discovery

Fig. 1: The D2 background did not alter protection conferred by Ddit3 deletion after axonal injury.

From: DDIT3 (CHOP) contributes to retinal ganglion cell somal loss but not axonal degeneration in DBA/2J mice

Fig. 1

a Retinal flat mounts 5 days post-CONC stained for the RGC marker RBPMS (green) and cleaved caspase 3 (cCASP3, red). There were significantly fewer cCASP3 + RGCs (cCASP3+ RBPMS+ cells) in D2.Ddit3−/− retinas compared with D2.Ddit3+/+ retinas (cCASP3+ RBPMS+ cells/mm2 ± SEM: D2.Ddit3+/+, 139.7 ± 7.5, D2.Ddit3−/−, 100.2 ± 13.8; n = 6 per genotype, P = 0.031, two-tailed t test). b Retinal flat mounts 14 days post-CONC stained for RBPMS. D2.Ddit3+/+ and D2.Ddit3−/− retinas had similar RGC densities 14 days post-sham surgery (RBPMS+ cells/mm2 ± SEM: D2.Ddit3+/+, 3811.4 ± 109.5, D2.Ddit3−/−, 3647.0 ± 166.2; n = 6 per genotype; P = 0.910, two-way ANOVA, Sidak post hoc). Both genotypes had a significant reduction of RGCs 14 days post-CONC (P < 0.001 for both comparisons, n = 6 per condition per genotype, two-way ANOVA, Sidak post hoc), however, D2.Ddit3−/− retinas had significantly more surviving RGC compared to D2.Ddit3+/+ controls (RBPMS+ cells/mm2 ± SEM: D2.Ddit3+/+, 991.0 ± 33.9, D2.Ddit3−/−, 1745.8 ± 116.5; n = 6 per condition per genotype; P = 0.001, two-way ANOVA, Sidak post hoc). Error bars, SEM. Scale bars, 100 μm

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