Fig. 5: High-glucose-induced miR-214-3p functionally targets β-catenin.

a Schematic diagram illustrating the design of luciferase reporters with the β-catenin 3′-UTR. The sequences of miR-214-3p and miR-214-3p-Mut are also shown. b The effect of AgomiR-214-3p, AgomiR-N.C and AgomiR-214-3p-Mut on luciferase activity in BMSCs after transfection with the β-catenin 3′-UTR reporter (n = 5). c The effect of AntagomiR-214-3p, AntagomiR-N.C and AntagomiR-214-3p-Mut on luciferase activity in BMSCs after transfection with the β-catenin 3′-UTR reporter (n = 5). d Western blot analysis of β-catenin protein levels in BMSCs after treatment with AgomiR-214-3p, AntagomiR-214-3p and their corresponding negative controls. e The effect of AntagomiR-214-3p and AntagomiR-N.C on luciferase activity in BMSCs supplemented with high glucose (n = 5). f Western blot analysis of β-catenin protein levels in BMSCs supplemented with high glucose after treatment with AntagomiR-214-3p or AntagomiR-N.C. g qRT-PCR analysis of Bglap and Alp in BMSCs after treatment with β-catenin siRNA and AntagomiR-214-3p in osteogenic medium supplemented with high glucose (n = 3). h Alizarin red staining of calcium deposition in BMSCs after treatment with β-catenin siRNA and AntagomiR-214-3p in osteogenic medium supplemented with high glucose. All data are expressed as mean ± SEM, **p < 0.01