Fig. 1: CD40 expression by normal (HRPT) and malignant (RCC) cells and its regulation by pro-inflammatory cytokines. | Cell Death Discovery

Fig. 1: CD40 expression by normal (HRPT) and malignant (RCC) cells and its regulation by pro-inflammatory cytokines.

From: CD40 induces renal cell carcinoma-specific differential regulation of TRAF proteins, ASK1 activation and JNK/p38-mediated, ROS-dependent mitochondrial apoptosis

Fig. 1

a Detection of CD40 expression in RCC lines ACHN, 786-O and A-704 and in normal HRPT cells by immunoblotting. Lysates from HCT116 and SW480 cell cultures served as CD40+ve and CD40-ve controls, respectively. Correct loading (20 µg protein/lane) was confirmed by β-actin detection. b Surface expression of CD40 by HRPT cells and RCC lines was examined by flow cytometry using CD40-PE (red histograms) in comparison to control PE-conjugated (black histograms) antibody. HCT116 and EJ cell lines served as CD40+ve controls. c RCC cells were treated with TNF-α (green histograms) or IFN-γ (purple histograms) for 48 h and CD40 expression was assessed alongside untreated cells by flow cytometry. Overlay histograms from a representative experiment are shown on the left and a summary of two independent experiments is shown on the right. Bars show mean median fluorescence intensity (MFI) of three technical replicates ± SEM.

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