Fig. 4: Effects of CDDP and/or ATO on 14-3-3ε/PI-3K/Akt signaling in CCA cells. | Cell Death Discovery

Fig. 4: Effects of CDDP and/or ATO on 14-3-3ε/PI-3K/Akt signaling in CCA cells.

From: Arsenic trioxide enhances the chemotherapeutic efficiency of cisplatin in cholangiocarcinoma cells via inhibiting the 14-3-3ε-mediated survival mechanism

Fig. 4

a CCA cells were treated with 0.0, 10.0, 20.0, or 80.0 μM of CDDP or with 0.0 to 2.0 μM of ATO for 24 h. Western blot (top) and quantitative analysis (bottom) of the expressions of 14-3-3ε. b and c HuCCT1 or RBE cells were treated with 10.0 μM of CDDP in the presence or absence of 2.0 μM of ATO for 12 (to determine the mRNAs) or 24 h (to determine the proteins). b Western blot (top) and quantitative analysis (bottom) of the expressions of 14-3-3ε or p-Akt. c qPCR analysis in triplicate of the Bcl-2 and survivin mRNAs. *p < 0.05 and **p < 0.01 vs. control group; ##p < 0.01 vs. CDDP-treated group.

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