Fig. 5: The inhibition of 14-3-3ε was involved in the ATO-enhanced chemotherapy efficiency of CDDP in CCA cells. | Cell Death Discovery

Fig. 5: The inhibition of 14-3-3ε was involved in the ATO-enhanced chemotherapy efficiency of CDDP in CCA cells.

From: Arsenic trioxide enhances the chemotherapeutic efficiency of cisplatin in cholangiocarcinoma cells via inhibiting the 14-3-3ε-mediated survival mechanism

Fig. 5

After HuCCT1 or RBE cells were transfected by NC- or 14–3-3ε-siRNA, they were treated with 10.0 μM of CDDP in the presence or absence of 2.0 μM of ATO. a Western blot (left, top) and quantitative analysis (left, bottom) of the expressions of γ-H2AX in HuCCT1 cells. The right graph was a statistical analysis of the extent of ATO-enhanced efficiency of CDDP (DNA damages, %). (b, top, and bottom, left) Percentage of cell apoptosis (Q2 + Q3). The right graph was a statistical analysis of the extent of ATO-enhanced efficiency of CDDP (apoptosis, %). c and d The cell viability was determined in triplicate, and the IC50s were calculated. **p < 0.01 vs. MOCK group; ##p < 0.01 vs. CDDP-treated group.

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