Fig. 4: Drp1 overexpression abolished the ACEA-induced effects on mitochondrial fission, fission-associated protein expression, and mitochondrial function in HT22 cells exposed to OGD/R. | Cell Death Discovery

Fig. 4: Drp1 overexpression abolished the ACEA-induced effects on mitochondrial fission, fission-associated protein expression, and mitochondrial function in HT22 cells exposed to OGD/R.

From: Cannabinoid CB1 receptor agonist ACEA alleviates brain ischemia/reperfusion injury via CB1–Drp1 pathway

Fig. 4

A Representative images of neuronal dendrites expressing Mito-DsRed, a mitochondrial marker. B Statistical results of the images in (A). C Western blot results showing Drp1, Fis1, and Mff expressions. D Drp1 overexpression (Drp1-OE) abolished the cannabinoid CB1 agonist ACEA-induced effect on Drp1 expression. E Drp1-OE abolished the ACEA-induced effect on Fis1 expression. F Drp1-OE abolished the ACEA-induced effect on Mff expression. G, H. Drp1-OE reversed the ACEA-induced effects on the activities of mitochondrial complexes I and IV in HT22 cells. Bar = 1 µm; *p < 0.05; NS no significance.

Back to article page