Fig. 1: Expression of macrophage colony-stimulating factor 1 receptor (M-CSF1-R) is increased in intestinal CD68 + HLA-DR + tumor-infiltrating cells (TICs).

A, B Representative dotplots showing M-CSF1-R-expressing cells in TICs or lamina propria mononuclear cells (LPMCs) isolated, respectively, from tumoral and non-tumoral areas of surgical samples of one patient with colon rectal cancer (CRC). Cells were gated on live CD45 + cells and subsequently analyzed for the expression of M-CSF1-R and CD3 (A) or CD19 (B) by flow cytometry. One of five representative experiments is shown. Staining with the respective isotype control antibodies is also shown. C Representative dotplots showing M-CSF1-R-expressing cells in TICs or LPMCs isolated as above. Cells were gated on CD45 + live cells and subsequently analyzed for the expression of M-CSF1-R and CD68 by flow cytometry. The right panel shows live CD45 + CD68 + cells expressing M-CSF1-R in TICs or LPMCs isolated from five patients with CRC. Each point in the graph indicates the percentage of live CD45 + CD68 + cells expressing M-CSF1-R in a single sample of a single CRC patient. Horizontal bars indicate the median values. D Intestinal TICs were isolated from tumoral areas of five patients with CRC, and M-CSF1-R expression was analyzed in live CD45 + CD68 + HLA-DRII + cells by flow cytometry. Each point in the graph indicates the percentage of live CD45 + CD68 + HLA-DRII + cells either expressing or not M-CSF1-R in a single sample of a single CRC patient. Horizontal bars indicate the median values. Right panel: representative histograms showing the expression of M-CSF1-R in TICs isolated from tumoral area of one patient with CRC and analyzed by flow cytometry. The numbers indicate the percentage of live CD45 + CD68 + HLA-DRII + cells, either positive or negative, for M-CSF1-R. Histograms with isotype control antibodies are also shown.