Fig. 4: Tumor-associated macrophages (TAMs) produce IL-34.
From: Macrophages produce and functionally respond to interleukin-34 in colon cancer
A, B Intestinal TICs and LPMCs were isolated, respectively, from tumoral and non-tumoral samples of six patients with CRC and analyzed for the percentage of live CD45 + CD68 + HLA-DRII + IL-34+ cells either expressing (A) or not (B) M-CSF1-R + by flow cytometry. Each point in the graph indicates the percentage of positive cells in a single sample of a single CRC patient; horizontal bars indicate the median values. Right panels: representative histograms showing IL-34 expression in live CD45 + CD68 + HLA-DRII + TICs and LPMCs either expressing (A) or not (B) M-CSF1-R. Staining with isotype control antibodies is also shown. C Representative photomicrographs (×200 original magnification) of serial formalin-fixed, paraffin-embedded colon sections of surgical samples taken from the tumoral area of one patient with CRC and stained with IL-34 or CD68. The right panel shows the correlation between the number of IL-34-expressing cells and the number of CD68-positive cells as evaluated by immunostaining in frozen sections of surgical specimens taken from five CRC patients.
