Fig. 1: PTTG3P is upregulated in PDAC tissues.

a In situ hybridization (ISH) analysis of PTTG3P was performed in 25 paired paraffin-embedded PDAC and adjacent tissues (CH cohort 1). Differential expression of PTTG3P in tumor and adjacent tissues was shown in a heat-map and was statistically analyzed by Wilcoxon signed rank tests. b Representative images are shown for absent, weak, moderate and strong expression of PTTG3P. Scale bars, 100 μm. c PTTG3P expression was analyzed by qRT-PCR in PDAC samples and adjacent non-tumor PDAC tissues from cohort 2 (N = 60). PTTG3P expression level was normalized to that of GAPDH. d ROC curve for prediction of PDAC using qRT-PCR-based PTTG3P expression level. The AUC was 0.838, with indicated P value. e and f. Expression of PTTG3P was positively correlated with grades of tumor differentiation (e) and tumor size (f). g Kaplan–Meier survival analysis of overall survival in PDAC patients in CH cohort 2. h UALCAN database showed high expression of PTTG3P indicates poor prognosis of PDAC patients in TCGA cohorts.